Chemical product testing instruments

Nanjing - Chemical Product Testing Instruments - Professional Testing Agency (Aikon Quanxin), Aikon Quanxin Testing Center currently possesses advanced domestic high-resolution 400M nuclear magnetic resonance spectrometers, a full set of Agilent series instruments including liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), gas chromatography (GC), and other large-scale analytical instruments. Here, we have a high-quality management team and an experienced technical team. The testing center will always be customer demand-oriented, continuously providing efficient, fast, intuitive, and reliable testing solutions to a wide range of clients with new industry standards and technical means, and is committed to offering personalized and consistent services to both new and existing customers.
Currently, the main commercial reagents used in chromatography include the following:
1. Stationary phases for gas chromatography
Common fillers such as supports, diatomaceous earth, porous microspheres, and molecular sieve series. The more commonly used varieties for analysis, the higher the degree of domestication. There are also pre-treated acid-washed or acid-washed silanized supports and stationary phases already coated with fixed liquids, such as Chromosorb, Porapak, and HayeSep series, most of which are imported. For columns with different requirements, the stationary phase should be selected with appropriate mesh sizes to achieve ideal separation and analysis purposes. With the development and changes in chromatography columns, especially the widespread application of capillary columns, the selection and use of chromatographic stationary liquids have correspondingly decreased. For example, domestically produced capillary electrophoresis instruments are gradually approaching international standards, achieving full-process automation. The development of various application kits for nucleic acid, protein, ion, and chiral drug analysis has also reduced the demand for common reagents. Currently, commonly used stationary liquids include methyl silicone oil, silicone OV series, polyethylene glycol series, phthalic acid ester series, and Carbowax series, as well as some silicone-modified reagents and chiral stationary liquids. Chromatographic standards and reference materials, typically with a purity greater than 99.5%, range from single molecules to multi-component standards such as petroleum, environmental, and pesticide residues. Most of these reagents are imported. Overall, foreign gas chromatographs tend to be more automated and computerized, while domestic instruments are more specialized and serialized, which places higher demands on reagents.
2. Liquid chromatography reagents
The most commonly used mobile phase reagents include methanol, acetonitrile, acetone, tetrahydrofuran, isopropanol, n-hexane, isooctane, and chloroform. Multiple technical indicators of the mobile phase, such as content, light absorption range, acidity and alkalinity, viscosity, moisture, color, fluorescence background, and LC gradient stability, affect the column's lifespan and the quality of test results. There are not many new models of liquid chromatographs, with HPILC instruments being the main type. In recent years, the development of capillary electrophoresis has had a significant impact on ion chromatography. In fact, ion chromatography (IC) and exchange chromatography (EC) are two different analytical techniques, each with its own advantages and disadvantages, and neither can completely replace the other. In metal ion analysis using ion chromatography, high-purity reagents are still used in certain quantities, though their varieties, specifications, and prices vary significantly. Additionally, foreign reagent catalogs have newly listed many ion-pair reagents for HPLC. For reagents related to HPLC, the main issue is that the demand for each variety is not high, but the quality requirements are stringent. If imported varieties are chosen, the price is not low.
3. Supercritical fluid chromatography and supercritical fluid extraction systems, medium-low pressure chromatography, and perfusion chromatography
These are relatively new types of high-performance liquid chromatography systems. SFE primarily uses pure CO2 as the extraction agent. If other organic solvents are added as modifiers, the effect is better. Perfusion chromatography is based on the patent of perfusion fillers. This technology uses highly cross-linked polyethylene divinylbenzene as the matrix, which, after appropriate treatment, obtains a dual-mode pore structure, giving the medium both diffusion pores and through-pores. During the separation process, the mobile phase can directly penetrate the medium particles. Based on the above perfusion chromatography fillers, using existing liquid chromatography filler surface bonding technology, bonding chromatographic ligands with different functional groups can yield various modes of chromatography fillers, such as reverse phase, ion exchange, hydrophobic, and affinity, to separate different types of samples. Looking ahead at the development of this technology, we can see that not only polyethylene divinylbenzene can serve as a perfusion chromatography matrix, but silica gel with ultra-large pores and through-pores, dextran, cellulose, and filter membranes may also serve as separation media based on similar principles. Therefore, with the needs of biological science, bioengineering, and the pharmaceutical industry, these new types of fast chromatography fillers will undoubtedly develop further.
4. Thin-layer chromatography
Commonly used materials for thin-layer chromatography include silica gel and fluorescent silica gel plates, microcrystalline cellulose, alumina, and polyamide. After chemical treatment, such as with NH2, AC, CM, DEAE, and other groups, they can be used for reverse-phase thin-layer chromatography.
Some reagent catalogs also compile reagents related to GC and HPLC, such as pre-treatment reagents for gas chromatography; reagents used for silanization, acylation, esterification, and alkylation; labeling reagents for HPILC, such as ultraviolet, fluorescence, and chemiluminescence detection reagents, optical purity determination reagents, and reagents for instrumental analysis like NMR.
Chromatographic reagents serve instrumental analysis. With the continuous development and improvement of scientific and technological levels, and the updating and improvement of various analytical instruments and detection methods, the demand for chromatographic reagents will inevitably undergo greater changes.
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